Fractionation of Tetrahymena ciliary membranes with triton X-114 and the identification of a ciliary membrane ATPase.
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Fractionation of Tetrahymena ciliary membranes with triton X-114 and the identification of a ciliary membrane ATPase
Cilia were isolated from Tetrahymena thermophila, extracted with Triton X-114, and the detergent-soluble membrane + matrix proteins separated into Triton X-114 aqueous and detergent phases. The aqueous phase polypeptides include a high molecular mass polypeptide previously identified as a membrane dynein, detergent-soluble alpha and beta tubulins, and numerous polypeptides distinct from those f...
متن کاملFractionation of Tetrahymena Ciliary Membranes with Triton X-U4 and the Identification of a Ciliary Membrane ATPase
Cilia were isolated from Tetrahymena thermophila, extracted with Triton X-114, and the detergent-soluble membrane + matrix proteins separated into Triton X-114 aqueous and detergent phases. The aqueous phase polypeptides include a high molecular mass polypeptide previously identified as a membrane dynein, detergent-soluble ct and 13 tubulins, and numerous polypeptides distinct from those found ...
متن کاملCiliary Membranes with Triton X-U4 and the Identification of a Ciliary Membrane ATPase
Cilia were isolated from Tetrahymena thermophila, extracted with Triton X-114, and the detergent-soluble membrane + matrix proteins separated into Triton X-114 aqueous and detergent phases. The aqueous phase polypeptides include a high molecular mass polypeptide previously identified as a membrane dynein, detergent-soluble ct and 13 tubulins, and numerous polypeptides distinct from those found ...
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We have examined thin sections and replicas of freeze-fractured cilia of Tetrahymena pyriformis. The ciliary necklace located at the base of all freeze-fractured oral and somatic cilia has been studied in thin sections. Since electron-dense linkers have been found to connect both microtubule doublets and triplets to the ciliary membrane at the level of the necklace, the linkers and the associat...
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The cortical microtubules determine how cellulose microfibrils are deposited in the plant cell wall and are thus important for the control of cell expansion. To understand how microtubules can control microfibril deposition, the components that link the microtubules to the plasma membrane (PM) of plant cells must be isolated. To obtain information on the properties of the tubulin-membrane assoc...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1988
ISSN: 0021-9525,1540-8140
DOI: 10.1083/jcb.107.6.2679